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school. “Paper 42” means Paper 4, variant 2.
Practice questions
Original questions written by HomiLabs in the style of the exam, with answers and mark schemes. They are not
Cambridge past paper questions and have not been reviewed by Cambridge. Try each one before opening the answer.
Q1Anaerobic respiration (yeast/muscle) · Core · Paper 1 style · 1 mark
(a)What are the products of anaerobic respiration in yeast?[1]
Show answer
(a) B alcohol (ethanol) and carbon dioxide
Yeast respires glucose anaerobically to ethanol and carbon dioxide. Lactic acid (A) is made by human muscle, not yeast.
Q2Anaerobic respiration (yeast/muscle) · Core · Paper 2 style · 3 marks
(a)Write the word equation for anaerobic respiration in human muscle cells.[1]
(b)State when muscle cells respire anaerobically.[1]
(c)Anaerobic respiration releases much less energy per glucose molecule than aerobic respiration. Suggest why.[1]
Show answer
(a) glucose → lactic acid
B1
(b) During vigorous exercise, when oxygen cannot be supplied fast enough for aerobic respiration.
B1 vigorous exercise / not enough oxygen.
(c) Glucose is only partly broken down; lactic acid still contains a lot of energy.
B1 incomplete breakdown of glucose.
Q3Anaerobic respiration (yeast/muscle) · Core · Paper 2 style · 3 marks
(a)Give three differences between aerobic respiration and anaerobic respiration in human muscle.[3]
Show answer
(a) Aerobic needs oxygen, anaerobic does not; aerobic produces carbon dioxide and water, anaerobic produces lactic acid; aerobic releases much more energy per glucose; aerobic is a complete breakdown of glucose, anaerobic is incomplete.
1 mark each for any three comparative differences.
Q4Anaerobic respiration (yeast/muscle) · Core · Paper 2 style · 3 marks
(a)State two ways in which humans use anaerobic respiration by yeast.[2]
(b)Name the gas produced by yeast that makes bread rise.[1]
Show answer
(a) Bread making (carbon dioxide makes dough rise); making ethanol/alcohol for biofuel or drinks.
1 mark each for any two.
(b) carbon dioxide
B1
Q5Anaerobic respiration (yeast/muscle) · Standard · Paper 1 style · 1 mark
(a)After a race, lactic acid is removed from the blood. In which organ is most of it broken down using oxygen?[1]
Show answer
(a) B liver
Blood carries lactic acid from the muscles to the liver, where it is broken down by aerobic respiration (paying back the oxygen debt). The kidneys (A) do not remove lactic acid this way.
Q6Anaerobic respiration (yeast/muscle) · Standard · Paper 2 style · 7 marks
A baker in Multan mixed flour, water, sugar and yeast to make 50 cm³ of dough. After 1 hour in a warm place the dough had a volume of 120 cm³.
(a)Calculate the percentage increase in volume of the dough.[2]
(b)Explain why the dough rises.[2]
(c)Explain why the dough was left in a warm place rather than a refrigerator.[2]
(d)State what happens to the alcohol made by the yeast when the dough is baked.[1]
Show answer
(a) 140 %
M1 (120 − 50) ÷ 50 × 100; A1 140 %.
(b) Yeast respires anaerobically, producing carbon dioxide gas; the bubbles are trapped in the dough, so it expands.
B1 yeast respiration produces carbon dioxide; B1 gas trapped / bubbles make dough expand.
(c) Warmth increases the activity of the yeast's enzymes, so respiration and carbon dioxide production are faster; in the cold enzymes work slowly.
B1 enzymes work faster at warm temperature (near optimum); B1 faster respiration / more gas.
(d) It evaporates (boils off) in the oven heat.
B1
Q7Anaerobic respiration (yeast/muscle) · Standard · Paper 2 style · 7 marks
An athlete in Islamabad ran a 400 m race. At the end of the race her blood lactic acid concentration was 12.0 mmol per dm³. After resting for 45 minutes it was 3.0 mmol per dm³.
(a)Calculate the mean rate at which the lactic acid concentration fell during the 45 minutes. Give your answer in mmol per dm³ per minute.[2]
(b)Explain why lactic acid built up in the athlete's blood during the race.[2]
(c)Describe how the oxygen debt is repaid after the race.[3]
Show answer
(a) 0.2 mmol per dm³ per minute
M1 (12.0 − 3.0) ÷ 45; A1 0.2.
(b) Muscles needed more energy than aerobic respiration could supply; oxygen could not reach them fast enough, so they respired anaerobically, producing lactic acid.
B1 not enough oxygen to muscles; B1 anaerobic respiration produces lactic acid.
(c) Heart rate stays high to carry lactic acid in the blood from the muscles to the liver; breathing stays fast and deep to supply oxygen; lactic acid is broken down by aerobic respiration in the liver.
B1 fast heart rate transports lactic acid to liver; B1 deep/fast breathing supplies extra oxygen; B1 aerobic respiration of lactic acid in liver.
Q8Anaerobic respiration (yeast/muscle) · Standard · Paper 4 style · 7 marks
Yeast was mixed with sugar solutions of different concentrations at 30 °C. Each tube was connected by a delivery tube to a tube of water, and the bubbles of gas were counted for one minute, three times.Sugar concentration / %: 0 | 2 | 4 | 6 | 8Bubbles per minute, count 1: 0 | 8 | 17 | 25 | 26Bubbles per minute, count 2: 0 | 10 | 19 | 24 | 27Bubbles per minute, count 3: 0 | 9 | 18 | 26 | 25
(a)Calculate the mean number of bubbles per minute for each sugar concentration.[2]
(b)Describe the effect of sugar concentration on the rate of respiration of the yeast.[2]
(c)Explain why the bubbles at 0 % sugar were zero.[1]
(d)Explain why counting bubbles is not an accurate way to measure the volume of gas, and suggest a better method.[2]
Show answer
(a) 2 %: 9.0; 4 %: 18.0; 6 %: 25.0; 8 %: 26.0
B2 all correct; B1 two or three correct.
(b) Rate increases as sugar concentration increases from 0 % to 6 %, then levels off between 6 % and 8 %.
B1 increase; B1 levels off with data.
(c) There is no glucose (substrate) for the yeast to respire, so no carbon dioxide is made.
B1
(d) Bubbles are different sizes; collect the gas in a gas syringe or an inverted measuring cylinder over water and measure its volume.
B1 bubbles vary in size / may be missed; B1 gas syringe / measuring cylinder.
Q9Anaerobic respiration (yeast/muscle) · Standard · Paper 4 style · 7 marks
Equal samples of bread dough, each 20 cm³, were placed in measuring cylinders in water baths at different temperatures. The volume was read after 30 minutes.Temperature / °C: 15 | 25 | 35 | 45 | 60Volume after 30 min / cm³: 24 | 31 | 40 | 34 | 20
(a)Calculate the increase in volume of the dough at each temperature.[2]
(b)Calculate the percentage increase in volume at 35 °C.[1]
(c)Explain why the dough at 60 °C did not rise.[2]
(d)State two variables that should be kept the same.[2]
Q11Anaerobic respiration (yeast/muscle) · Challenge · Paper 4 style · 6 marks
A flask of glucose solution and yeast, closed with a cotton wool plug, was placed on a top-pan balance at 30 °C.Time / min: 0 | 15 | 30 | 45 | 60Mass of flask / g: 250.00 | 249.86 | 249.62 | 249.36 | 249.12Assume that all the mass lost is carbon dioxide released by anaerobic respiration of the yeast.
(a)Calculate the total mass of carbon dioxide lost in 60 minutes.[1]
(b)Calculate the mean rate of mass loss over the 60 minutes, in g per minute, to 3 significant figures.[1]
(c)Use the equation C₆H₁₂O₆ → 2C₂H₅OH + 2CO₂ to calculate the mass of glucose respired. (Mr: glucose 180, CO₂ 44)[2]
(d)Explain why the cotton wool plug was used instead of a rubber bung.[2]
Show answer
(a) 0.88 g
250.00 − 249.12 = 0.88 g. B1
(b) 0.0147 g per minute
0.88 ÷ 60 = 0.0147 g/min. B1
(c) 1.80 g
M1 180 g glucose → 2 × 44 = 88 g CO₂; A1 0.88 × 180 ÷ 88 = 1.80 g.
(d) It lets the carbon dioxide escape, so the loss in mass can be measured; it still keeps out microorganisms (and limits air movement), while a bung would trap the gas (and could be forced out by the pressure).
B1 lets carbon dioxide escape (mass loss measured); B1 keeps out microorganisms / bung would trap gas and build up pressure.
Q12Anaerobic respiration (yeast/muscle) · Challenge · Paper 2 style · 6 marks
At a distillery near a sugar mill in Sindh, molasses (a sugary waste from sugar cane) is diluted and mixed with yeast in closed tanks to make ethanol, which is sold as a fuel.
(a)Explain why air is kept out of the fermentation tanks.[2]
(b)Explain why the temperature of the tanks is controlled at about 30–35 °C.[2]
(c)Fermentation stops after some days even though the yeast is still present. Suggest two reasons.[2]
Show answer
(a) With oxygen, yeast would respire aerobically, producing carbon dioxide and water instead of ethanol; without oxygen it respires anaerobically and makes ethanol.
B1 aerobic respiration with oxygen gives no ethanol; B1 anaerobic conditions make ethanol.
(b) This is near the optimum for yeast enzymes, so respiration is fast; higher temperatures would denature the enzymes / kill the yeast.
B1 optimum for enzymes; B1 high temperature denatures / kills yeast.
(c) The sugar is used up; the ethanol concentration becomes high enough to poison/kill the yeast.
B1 sugar used up; B1 ethanol toxic to yeast (or pH falls).
Q13Anaerobic respiration (yeast/muscle) · Challenge · Paper 2 style · 5 marks
A student's breathing rate was recorded at rest and after 3 minutes of skipping.At rest: 14 breaths per minuteEnd of exercise: 382 minutes after: 305 minutes after: 2210 minutes after: 15
(a)Calculate the percentage increase in breathing rate from rest to the end of the exercise.[2]
(b)Explain why the breathing rate stayed above the resting rate for several minutes after exercise.[3]
Show answer
(a) 171 %
M1 (38 − 14) ÷ 14 × 100; A1 171 %.
(b) An oxygen debt built up because muscles respired anaerobically and made lactic acid; extra oxygen is needed to break down the lactic acid by aerobic respiration (in the liver); so fast, deep breathing continues until the lactic acid is removed.
B1 anaerobic respiration / lactic acid formed; B1 oxygen debt; B1 extra oxygen used to break down lactic acid (aerobically, in liver).
Q14Respiration investigations & respirometers · Core · Paper 4 style · 5 marks
Two vacuum flasks were set up with a thermometer in each.Flask A: germinating peas – 21.0 °C at the start, 26.5 °C after 3 daysFlask B: boiled peas – 21.0 °C at the start, 21.0 °C after 3 days
(a)Calculate the temperature rise in flask A.[1]
(b)Explain why the temperature in flask A increased.[2]
(c)Explain the purpose of flask B.[1]
(d)Both sets of peas were rinsed in dilute disinfectant. Suggest why.[1]
Show answer
(a) 5.5 °C
26.5 − 21.0 = 5.5 °C. B1
(b) The germinating peas respire, releasing energy, some as heat; the vacuum flask stops the heat escaping.
B1 respiration releases energy as heat; B1 flask insulates.
(c) A control: boiled peas are dead and do not respire, showing the heat comes from respiration of living peas.
B1
(d) To kill microorganisms (bacteria, fungi) on the peas, whose respiration would also release heat.
B1
Q15Respiration investigations & respirometers · Core · Paper 4 style · 3 marks
Air was drawn slowly through two tubes and then bubbled through limewater.Tube X: germinating peasTube Y: the same volume of glass beads
(a)State what happens to the limewater in tube X.[1]
(b)Explain your answer to (a).[1]
(c)State why glass beads were used in tube Y.[1]
Show answer
(a) It turns milky (cloudy).
B1
(b) The germinating peas respire and release carbon dioxide.
B1
(c) As a control with the same volume of solid but no living material.
B1
Q16Respiration investigations & respirometers · Core · Paper 1 style · 1 mark
(a)Which observation shows that carbon dioxide is present?[1]
Show answer
(a) A limewater turns milky
Carbon dioxide turns limewater milky. Cobalt chloride paper (C) tests for water, and indicator going purple (B) shows less carbon dioxide.
Q17Respiration investigations & respirometers · Standard · Paper 1 style · 1 mark
(a)In a respirometer containing germinating seeds and soda lime, a drop of coloured liquid in the capillary tube moves towards the seeds. Why?[1]
Show answer
(a) B The seeds take in oxygen and the carbon dioxide they release is absorbed by the soda lime.
Oxygen is used and the carbon dioxide released is absorbed, so the gas volume falls and the drop moves towards the seeds. A is wrong because the carbon dioxide is removed by the soda lime.
Q18Respiration investigations & respirometers · Standard · Paper 4 style · 5 marks
Eight woodlice were placed in a respirometer containing soda lime, kept in a water bath at 25 °C. The coloured drop in the capillary tube (internal radius 0.5 mm) moved 24 mm in 5 minutes.
(a)Calculate the volume of oxygen used in 5 minutes. Use volume = πr²l, where l is the distance moved. Give your answer to 1 decimal place.[2]
(b)Calculate the rate of oxygen use in mm³ per minute, to 2 decimal places.[1]
(c)State the role of the soda lime.[1]
(d)Suggest why the apparatus was left for 10 minutes in the water bath before the readings were started.[1]
Show answer
(a) 18.8 mm³
M1 π × 0.5² × 24; A1 18.8 mm³ (18.85).
(b) 3.77 mm³ per minute
18.85 ÷ 5 = 3.77. B1
(c) It absorbs the carbon dioxide released by the woodlice.
B1
(d) To let the temperature of the air inside reach that of the water bath, so expansion/contraction of air does not move the drop.
B1 temperature equilibration.
Q19Respiration investigations & respirometers · Standard · Paper 4 style · 6 marks
Hydrogencarbonate indicator is red in normal air, yellow when carbon dioxide concentration is high and purple when it is low. Four sealed tubes, kept in the dark, each contained 5 cm³ of red indicator and a gauze platform holding:A: ten maggotsB: glass beadsC: germinating mung bean seedsD: boiled mung bean seeds
(a)Predict the colour of the indicator in each tube after 2 hours.[4]
(b)Explain the colour in tube C.[2]
Show answer
(a) A yellow; B red; C yellow; D red
B1 each.
(b) Germinating seeds respire, releasing carbon dioxide, which makes the indicator more acidic, so it turns yellow.
Q20Respiration investigations & respirometers · Standard · Paper 4 style · 5 marks
Yeast in glucose solution was connected to a gas syringe at 35 °C.Time / min: 0 | 2 | 4 | 6 | 8 | 10Volume of gas / cm³: 0 | 6 | 14 | 22 | 27 | 30
(a)Calculate the mean rate of gas production between 2 and 6 minutes, in cm³ per minute.[2]
(b)Describe how the rate changes after 6 minutes and suggest why.[2]
(c)Name the gas collected.[1]
Show answer
(a) 4.0 cm³ per minute
M1 (22 − 6) ÷ 4; A1 4.0.
(b) The rate slows (only 8 cm³ in the last 4 minutes) because the glucose is being used up / ethanol builds up.
B1 rate decreases with data; B1 substrate used up / product inhibits.
(c) carbon dioxide
B1
Q21Respiration investigations & respirometers · Challenge · Paper 4 style · 8 marks
A respirometer with 2 g of fly larvae and soda lime was placed in water baths at different temperatures. The distance moved by the drop in 10 minutes was recorded.Temperature / °C: 10 | 15 | 20 | 25 | 30Distance in 10 min / mm: 6 | 11 | 17 | 9 | 34
(a)Identify the anomalous result and suggest a reason for it.[2]
(b)Calculate the rate of movement of the drop at 30 °C in mm per minute.[1]
(c)Calculate how many times greater the distance was at 30 °C than at 10 °C, to 1 decimal place.[1]
(d)Explain why the rate of respiration increased with temperature.[2]
(e)Suggest two improvements to make the results more reliable.[2]
Show answer
(a) 25 °C (9 mm) – expected about 23–25 mm; a leak in the apparatus / the larvae were not at the bath temperature / fewer larvae used.
B1 25 °C value; B1 sensible reason.
(b) 3.4 mm per minute
34 ÷ 10 = 3.4. B1
(c) 5.7
34 ÷ 6 = 5.7. B1
(d) Respiration is controlled by enzymes; higher temperature gives molecules more kinetic energy so more collisions / enzyme–substrate complexes, until the optimum.
B1 enzymes; B1 more kinetic energy / collisions.
(e) Repeat each temperature three times and calculate means; use the same mass of larvae; leave to equilibrate before timing; use a thermostatic water bath.
1 mark each, max 2.
Q22Respiration investigations & respirometers · Challenge · Paper 4 style · 6 marks
You are provided with dried yeast, 5 % glucose solution, boiling tubes with delivery tubes, gas syringes, water baths, thermometers, a stopwatch and vegetable oil.
(a)Plan an investigation to find the effect of temperature on the rate of anaerobic respiration of yeast.[6]
Show answer
(a) A plan with at least 5 temperatures, a measured product, controlled variables, repeats and a fair way to compare.
1 mark each, max 6: use water baths at e.g. 20, 30, 40, 50, 60 °C; same mass of yeast and same volume and concentration of glucose solution; leave to reach temperature before mixing; a layer of oil on top (or sealed) to keep oxygen out; measure carbon dioxide volume in a gas syringe (or count bubbles) for a fixed time, e.g. 10 min; repeat three times and calculate mean; rate = volume ÷ time; control of boiled yeast; risk: hot water / care with glassware.
Q23Respiration investigations & respirometers · Challenge · Paper 2 style · 5 marks
Twenty wheat grains had a total dry mass of 5.40 g. Twenty similar grains were germinated in the dark for 6 days, then dried in an oven; their dry mass was 4.32 g.
(a)Calculate the percentage decrease in dry mass.[2]
(b)Explain why the dry mass decreased.[2]
(c)Explain why dry mass, rather than fresh mass, was measured.[1]
Show answer
(a) 20 %
M1 (5.40 − 4.32) ÷ 5.40 × 100; A1 20 %.
(b) The seeds respire, breaking down stored food (starch) to release energy; carbon dioxide (and water) is lost, and in the dark no photosynthesis replaces the food.
B1 respiration uses stored food; B1 carbon lost as CO₂ / no photosynthesis in dark.
(c) Fresh mass changes with the amount of water taken up, which does not show the change in food/organic material.
B1
Q24Aerobic respiration · Core · Paper 1 style · 1 mark
(a)In which part of a cell does most aerobic respiration take place?[1]
Show answer
(a) B mitochondria
Aerobic respiration releases energy in the mitochondria. Ribosomes (C) make proteins.
Q25Aerobic respiration · Core · Paper 2 style · 4 marks
(a)Write the word equation for aerobic respiration.[2]
(b)Write the balanced chemical equation for aerobic respiration.[2]
Show answer
(a) glucose + oxygen → carbon dioxide + water
B1 reactants; B1 products.
(b) C₆H₁₂O₆ + 6O₂ → 6CO₂ + 6H₂O
B1 formulae; B1 balanced.
Q26Aerobic respiration · Standard · Paper 1 style · 1 mark
(a)Which process in the human body does NOT need energy from respiration?[1]
Show answer
(a) C diffusion of oxygen into the blood in the alveoli
Diffusion is passive and needs no energy from respiration. Active transport (B) needs energy, so it is a distractor.
Q27Aerobic respiration · Standard · Paper 2 style · 6 marks
(a)State four uses of the energy released by respiration in the human body.[4]
(b)Define aerobic respiration.[2]
Show answer
(a) Any four: muscle contraction; protein synthesis; cell division; active transport; growth; passage of nerve impulses; maintaining a constant body temperature.
1 mark each.
(b) The chemical reactions in cells that use oxygen to break down nutrient molecules (glucose) to release energy.
B1 uses oxygen; B1 breaks down nutrients/glucose to release energy (in cells).
Q28Aerobic respiration · Standard · Paper 2 style · 5 marks
(a)Muscle cells in the legs of a cyclist contain many mitochondria. Explain why.[2]
(b)The middle piece of a sperm cell is packed with mitochondria. Suggest why.[1]
(c)Name the two substances that the blood must bring to muscle cells for aerobic respiration.[2]
Show answer
(a) Mitochondria are the site of aerobic respiration; muscle contraction needs a lot of energy, so many mitochondria release more energy.
B1 aerobic respiration in mitochondria; B1 lots of energy needed for contraction.
(b) To release energy for the tail (flagellum) to move the sperm.
B1
(c) glucose and oxygen
B1 each.
Q29Aerobic respiration · Challenge · Paper 2 style · 4 marks
B1 water = 1.2 × 18 = 21.6 g and totals equal (74.4 g).
Q30Aerobic respiration · Challenge · Paper 2 style · 5 marks
For this question, assume that aerobic respiration releases 16.0 kJ per gram of glucose and anaerobic respiration in muscle releases 0.8 kJ per gram of glucose.
(a)Calculate how many times more energy is released per gram of glucose by aerobic respiration than by anaerobic respiration.[1]
(b)Calculate the mass of glucose needed to release 400 kJ by (i) aerobic respiration and (ii) anaerobic respiration.[2]
(c)Use these figures to explain why muscles cannot rely on anaerobic respiration for long periods.[2]
Show answer
(a) 20 times
16.0 ÷ 0.8 = 20. B1
(b) (i) 25 g; (ii) 500 g
B1 400 ÷ 16.0 = 25 g; B1 400 ÷ 0.8 = 500 g.
(c) Anaerobic respiration releases far less energy per gram, so glucose would be used up very quickly; lactic acid also builds up and causes fatigue.
B1 little energy / glucose used rapidly; B1 lactic acid accumulates / oxygen debt.
Similar questions
Questions that test the same subtopic in different years, grouped so you can work on one skill at a time.
References only: find each question in its paper (official link where available, otherwise through your school).