References only, not question text. Open Official paper ↗ where Cambridge publishes the paper,
or see the official Cambridge past-papers page; for other papers, ask your
school. “Paper 42” means Paper 4, variant 2.
Practice questions
Original questions written by HomiLabs in the style of the exam, with answers and mark schemes. They are not
Cambridge past paper questions and have not been reviewed by Cambridge. Try each one before opening the answer.
Q1Microscopy & magnification · Core · Paper 1 style · 1 mark
(a)[1]
Show answer
(a) C 90 µm
Actual size = image size ÷ magnification = 36 mm ÷ 400 = 0.09 mm = 90 µm (× 1000). A (0.09 µm) forgets to convert mm to µm.
Q2Microscopy & magnification · Core · Paper 1 style · 1 mark
(a)Which equation is correct for an image seen through a microscope?[1]
Show answer
(a) C image size = actual size × magnification
magnification = image size ÷ actual size, so image size = actual size × magnification. B is the formula upside down, which gives a value less than 1 for an enlargement.
Q3Microscopy & magnification · Core · Paper 4 style · 3 marks
A student peeled the thin inner layer from an onion scale leaf, stained it with iodine solution and drew one cell as seen with a light microscope.
(a)Line XY across the drawing of one cell measures 96 mm. The drawing has a magnification of ×120. Calculate the actual length of the cell in mm. Show your working.[2]
(b)Convert your answer to (a) into micrometres (µm).[1]
Show answer
(a) 0.8 mm
M1 actual = image ÷ magnification = 96 ÷ 120; A1 0.8 mm
(b) 800 µm
B1 × 1000 (1 mm = 1000 µm)
Q4Microscopy & magnification · Core · Paper 2 style · 3 marks
Paramecium is a single-celled organism found in pond water in Lahore. A specimen is 0.18 mm long. Its image in a field guide is 54 mm long.
(a)Calculate the magnification of the image. Show your working.[2]
(b)Express the actual length of the Paramecium in µm.[1]
Show answer
(a) ×300
M1 54 ÷ 0.18 (both in mm); A1 ×300
(b) 180 µm
B1 0.18 × 1000
Q5Microscopy & magnification · Core · Paper 4 style · 6 marks
A student in Peshawar wants to look at onion epidermis cells with a light microscope.
(a)Describe how the student should prepare a slide of onion epidermis so that cells can be seen clearly.[4]
(b)Explain why iodine solution is added.[1]
(c)State why the layer of tissue must be very thin.[1]
Show answer
(a) Peel a thin layer; place flat in a drop of water or iodine on the slide; lower a coverslip at an angle with a mounted needle; remove excess liquid with paper towel.
1 mark each for any four of: peel a thin (one cell thick) layer of epidermis with forceps; place it flat on a clean slide; add a drop of water / iodine solution; lower coverslip at an angle / from one side using a mounted needle; to avoid air bubbles; blot excess liquid
(b) It stains structures (e.g. the nucleus and cytoplasm) so they are easier to see.
B1 stains / increases contrast
(c) So that light can pass through it / cells do not overlap.
B1
Q6Microscopy & magnification · Core · Paper 2 style · 3 marks
(a)State the formula that links magnification, image size and actual size.[1]
(b)A chloroplast is 5 µm long. In a diagram it is drawn 20 mm long. Calculate the magnification of the diagram.[2]
Show answer
(a) magnification = image size ÷ actual size
B1
(b) ×4000
M1 20 mm = 20000 µm; A1 20000 ÷ 5 = ×4000
Q7Microscopy & magnification · Core · Paper 4 style · 3 marks
A student draws a root hair cell from a radish seedling at a magnification of ×40. The drawing of the cell, including its hair, is 18 mm long.
(a)Calculate the actual length of the root hair cell in mm.[2]
(b)State the actual length in µm.[1]
Show answer
(a) 0.45 mm
M1 18 ÷ 40; A1 0.45 mm
(b) 450 µm
B1
Q8Microscopy & magnification · Standard · Paper 1 style · 1 mark
(a)[1]
Show answer
(a) C ×2000
Convert to the same units: 50 mm = 50000 µm. Magnification = 50000 ÷ 25 = ×2000. B (×200) comes from a wrong conversion (1 mm = 100 µm).
Q9Microscopy & magnification · Standard · Paper 4 style · 5 marks
A photomicrograph shows a sunflower pollen grain at a magnification of ×850. The diameter of the pollen grain on the photograph is 34 mm.
(a)Calculate the actual diameter of the pollen grain in µm. Show your working.[2]
(b)A student makes a large drawing of the same pollen grain with a diameter of 68 mm. Calculate the magnification of the student's drawing.[2]
(c)State why a magnification has no units.[1]
Show answer
(a) 40 µm
M1 34 mm = 34000 µm, ÷ 850; A1 40 µm
(b) ×1700
M1 68000 µm ÷ 40 µm; A1 ×1700 (no units)
(c) It is a ratio of two lengths measured in the same units, so the units cancel.
B1 ratio / same units cancel
Q10Microscopy & magnification · Standard · Paper 4 style · 3 marks
A mosquito larva collected from a water tank is 12 mm long. A student makes a drawing of the larva that is 132 mm long.
(a)Calculate the magnification of the drawing. Show your working.[2]
(b)Another student got an answer of ×0.09. Explain how you know this answer must be wrong.[1]
Show answer
(a) ×11
M1 132 ÷ 12; A1 ×11
(b) The drawing is larger than the larva, so the magnification must be greater than 1 (the student divided actual size by drawing size).
B1 enlargement must be > 1 / divided the wrong way round
Q11Microscopy & magnification · Standard · Paper 2 style · 3 marks
A highly magnified image of a rod-shaped bacterium from yoghurt is 60 mm long. The magnification of the image is ×30000.
(a)Calculate the actual length of the bacterium in µm. Show your working.[2]
(b)How many of these bacteria, placed end to end, would fit across a line 1 mm long?[1]
Show answer
(a) 2 µm
M1 60 mm = 60000 µm, ÷ 30000; A1 2 µm
(b) 500
B1 1000 µm ÷ 2 µm = 500
Q12Microscopy & magnification · Standard · Paper 4 style · 4 marks
A student measured the lengths of five cheek cells on a photograph taken at ×1000. The image lengths were: 58 mm, 62 mm, 55 mm, 61 mm, 64 mm.
(a)Calculate the mean image length.[1]
(b)Use your mean to calculate the actual length of a typical cheek cell in µm.[2]
(c)Explain why the student measured five cells rather than one.[1]
Show answer
(a) 60 mm
B1 (58+62+55+61+64) ÷ 5 = 60 mm
(b) 60 µm
M1 60 mm ÷ 1000; A1 60 µm
(c) Cells vary in size, so a mean of several is more representative / reliable.
B1
Q13Microscopy & magnification · Standard · Paper 4 style · 3 marks
A student wants to draw a fish egg that is 0.8 mm in diameter at a magnification of ×50.
(a)Calculate how long the student's drawing of the egg should be.[2]
(b)State one feature of a good biological drawing that the student should include.[1]
Show answer
(a) 40 mm
M1 image = actual × magnification = 0.8 × 50; A1 40 mm
(b) Clear continuous outline lines / no shading / correct proportions / drawing large enough / labels with ruled lines.
B1 any one
Q14Microscopy & magnification · Challenge · Paper 1 style · 1 mark
(a)[1]
Show answer
(a) B 45 mm
Image size = actual size × magnification = 7.5 µm × 6000 = 45000 µm = 45 mm. A divides by 10 000 instead of 1000; D divides magnification by size.
Q15Microscopy & magnification · Challenge · Paper 4 style · 5 marks
A student draws a section through a mango leaf. The actual thickness of the leaf is 0.25 mm. On the drawing the thickness is 75 mm.
(a)Calculate the magnification of the drawing.[2]
(b)On the same drawing one palisade mesophyll cell is 21 mm long. Use your answer to (a) to calculate the actual length of this cell in µm.[2]
(c)The student's calculated value is larger than the value given in a textbook. Suggest one reason, other than a calculation error, for this difference.[1]
Show answer
(a) ×300
M1 75 ÷ 0.25; A1 ×300
(b) 70 µm
M1 21 ÷ 300 = 0.07 mm; A1 × 1000 = 70 µm
(c) The drawing was not drawn exactly to scale / the cells were drawn too large in proportion to the leaf thickness, or the leaf used was thicker / different cells vary in size.
B1 any one sensible reason
Q16Microscopy & magnification · Challenge · Paper 2 style · 5 marks
Two cells were photographed. Cell P (a cheek cell) has an image 45 mm long at ×1500. Cell Q (a plant cell) has an image 30 mm long at ×600.
(a)Calculate the actual length of each cell in µm.[3]
(b)A student says that cell P is bigger because its image is bigger. Use your answers to explain why the student is wrong.[2]
Show answer
(a) Cell P: 30 µm; cell Q: 50 µm
M1 convert mm to µm (× 1000); A1 P = 45000 ÷ 1500 = 30 µm; A1 Q = 30000 ÷ 600 = 50 µm
(b) Cell Q is actually larger, by 20 µm; image size depends on magnification as well as actual size.
B1 Q is larger (by 20 µm); B1 P was magnified more so its image is larger even though the cell is smaller
Q17Microscopy & magnification · Challenge · Paper 2 style · 5 marks
A human egg cell has a diameter of about 70 µm. A photograph shows an egg cell with an image diameter of 42 mm.
(a)Calculate the magnification of the photograph.[2]
(b)The same photograph is enlarged on a classroom screen so that the image of the cell becomes 210 mm long. Calculate the new magnification.[2]
(c)Explain whether enlarging the photograph lets you see more detail of the cell.[1]
Show answer
(a) ×600
M1 42 mm = 42000 µm; A1 42000 ÷ 70 = ×600
(b) ×3000
M1 210 000 µm ÷ 70 µm; A1 ×3000 (or 600 × 5)
(c) No — enlarging the photograph only makes the same image bigger; no new detail is revealed (resolution does not change).
B1
Q18Cell structure & organelles · Core · Paper 1 style · 1 mark
(a)Which structure is found in both plant cells and animal cells?[1]
Show answer
(a) D ribosome
Ribosomes (protein synthesis) occur in all plant and animal cells. Cell walls, chloroplasts and a large permanent vacuole are found in plant cells only.
Q19Cell structure & organelles · Core · Paper 2 style · 5 marks
(a)Copy and complete the table by writing 'present' or 'absent' in each space.Structure | palisade mesophyll cell | liver cellcell wall | ? | ?chloroplasts | ? | ?nucleus | ? | ?cell membrane | ? | ?[4]
(c) Gives the cell a fixed shape / support / stops the cell bursting when it takes in water.
B1
Q21Cell structure & organelles · Standard · Paper 1 style · 1 mark
(a)[1]
Show answer
(a) A aerobic respiration
Mitochondria are the site of aerobic respiration. Photosynthesis happens in chloroplasts, so B is wrong; diffusion needs no energy from respiration.
Q22Cell structure & organelles · Standard · Paper 2 style · 5 marks
Lactobacillus is a bacterium used to make yoghurt (dahi). Its cell has a cell wall, cell membrane, cytoplasm, ribosomes, a loop of circular DNA and small plasmids.
(a)State two structures that are found in this bacterial cell but are not found in a human cheek cell.[2]
(b)Describe how the genetic material of a bacterium differs from that of a plant cell.[2]
(c)Name the structures in the bacterium where proteins are made.[1]
Show answer
(a) Cell wall; plasmids (also accept circular DNA free in cytoplasm).
1 mark each for any two of: cell wall, plasmid(s), circular DNA
(b) Bacterium: a single circular DNA loop free in the cytoplasm (plus plasmids), not inside a nucleus. Plant cell: DNA in chromosomes inside a nucleus.
B1 no nucleus / DNA free in cytoplasm; B1 circular DNA / plasmids vs chromosomes in nucleus
(c) Ribosomes
B1
Q23Cell structure & organelles · Standard · Paper 2 style · 4 marks
(a)State the function of the nucleus.[1]
(b)State the function of ribosomes.[1]
(c)Describe the function of the large permanent vacuole in a plant cell.[2]
Show answer
(a) Contains genetic material (DNA / chromosomes) that controls the activities of the cell.
B1
(b) Protein synthesis.
B1
(c) Stores cell sap (water, sugars, salts); when full it presses the cytoplasm against the cell wall, keeping the cell turgid and supporting the plant.
B1 stores cell sap; B1 maintains turgor / support
Q24Cell structure & organelles · Standard · Paper 2 style · 4 marks
Cell R was found in a water sample from a pond in Murree. It has a nucleus, a cell wall, a cell membrane, several chloroplasts and a large vacuole full of cell sap.
(a)Identify whether cell R is a plant cell, an animal cell or a bacterial cell. Give two reasons from the description.[3]
(b)Cell S has a nucleus, cell membrane and many mitochondria but no cell wall. Name the type of cell.[1]
Show answer
(a) Plant cell: it has chloroplasts and a large permanent vacuole, and it has a cell wall as well as a nucleus.
B1 plant; 1 mark each for any two of: chloroplasts; large (permanent) vacuole containing cell sap; nucleus together with a cell wall (bacteria have a cell wall but no nucleus; animal cells have no cell wall)
(b) Animal cell
B1
Q25Cell structure & organelles · Challenge · Paper 2 style · 5 marks
(a)Suggest why sperm cells have many mitochondria in the middle region.[2]
(b)Root hair cells do not have chloroplasts. Explain why.[2]
(c)Suggest why cells in the pancreas that make digestive enzymes have many ribosomes.[1]
Show answer
(a) To carry out a lot of aerobic respiration, releasing energy for the tail to move / swim to the egg.
B1 aerobic respiration releases energy; B1 for tail movement / swimming
(b) They are underground so receive no light; photosynthesis cannot occur, so chloroplasts would be of no use.
B1 no light underground; B1 so no photosynthesis
(c) Enzymes are proteins, and ribosomes are where proteins are made.
B1
Q26Cell structure & organelles · Challenge · Paper 2 style · 5 marks
(a)State where in the cell the chemical reactions of aerobic respiration take place.[1]
(b)Muscle cells in a sprinter's legs contain more mitochondria than skin cells. Explain why.[2]
(c)Name the part of the cell where most chemical reactions of the cell take place, and state what it contains.[2]
Show answer
(a) Mitochondria
B1
(b) Muscle contraction needs a lot of energy; more mitochondria allow more aerobic respiration to release this energy.
B1 more aerobic respiration; B1 more energy (released) for contraction
(c) Cytoplasm; contains water, dissolved substances / enzymes and organelles.
B1 cytoplasm; B1 any one valid content
Q27Specialised cells & levels of organisation · Core · Paper 1 style · 1 mark
(a)Which list is in order of increasing level of organisation?[1]
Show answer
(a) B cell → tissue → organ → organ system
Cells form tissues, tissues form organs, organs form organ systems. A puts organ before tissue.
Q28Specialised cells & levels of organisation · Core · Paper 2 style · 5 marks
(a)For each of the following, state whether it is a cell, a tissue, an organ or an organ system: (i) the heart (ii) xylem (iii) a neurone (iv) the stomach, intestines and liver working together.[4]
(b)Define the term organ.[1]
Show answer
(a) (i) organ (ii) tissue (iii) cell (iv) organ system
1 mark each
(b) A group of tissues working together to perform specific functions.
B1
Q29Specialised cells & levels of organisation · Standard · Paper 2 style · 5 marks
(a)Copy and complete the table to show one feature of each cell and how it helps the cell's function.Cell | function | feature | how the feature helpsciliated cell | ? | ? | ?red blood cell | ? | ? | ?[4]
(b)State the meaning of the term tissue.[1]
Show answer
(a) Ciliated cell: moves mucus (and trapped particles) in the trachea/bronchi; cilia beat to move mucus. Red blood cell: transports oxygen; contains haemoglobin which binds oxygen / no nucleus so more room / biconcave shape gives large surface area.
Ciliated: B1 function (move mucus), B1 feature + explanation (cilia beat / sweep). RBC: B1 function (carry oxygen), B1 feature + explanation (haemoglobin / no nucleus / biconcave)
(b) A group of cells with similar structures, working together to perform a shared function.
B1
Q30Specialised cells & levels of organisation · Challenge · Paper 2 style · 5 marks
(a)Explain how the shape of a root hair cell helps it to absorb water and mineral ions.[2]
(b)Explain how a palisade mesophyll cell is adapted for photosynthesis.[2]
(c)A motor neurone can be over 1 m long. Suggest how this length is an advantage.[1]
Show answer
(a) The long hair-like extension gives a large surface area, so more water and ions can be absorbed at a time.
B1 long extension / hair; B1 large surface area for absorption
(b) It contains many chloroplasts to absorb light; it is long/column-shaped and near the upper surface so it receives more light.
1 mark each for any two: many chloroplasts; near upper surface; elongated shape packed closely; large vacuole pushes chloroplasts to edge
(c) It carries impulses over long distances (from the spinal cord to muscles) quickly without needing synapses along the way.
B1
Similar questions
Questions that test the same subtopic in different years, grouped so you can work on one skill at a time.
References only: find each question in its paper (official link where available, otherwise through your school).