References only, not question text. Open Official paper ↗ where Cambridge publishes the paper,
or see the official Cambridge past-papers page; for other papers, ask your
school. “Paper 42” means Paper 4, variant 2.
Practice questions
Original questions written by HomiLabs in the style of the exam, with answers and mark schemes. They are not
Cambridge past paper questions and have not been reviewed by Cambridge. Try each one before opening the answer.
Q1Chemical digestion & enzymes · Core · Paper 2 style · 1 mark
(a)Which enzyme begins the digestion of protein in the stomach?[1]
Show answer
(a) D pepsin
Pepsin is the protease secreted in the stomach and works at acidic pH. Amylase digests starch, not protein.
Q2Chemical digestion & enzymes · Core · Paper 4 style · 3 marks
(a)State the substrate and the products of the enzyme lipase.[2]
(b)Name the organ that secretes lipase into the duodenum.[1]
Show answer
(a) substrate: fats (lipids); products: fatty acids and glycerol
B1 fat; B1 fatty acids and glycerol (both).
(b) pancreas
B1.
Q3Chemical digestion & enzymes · Core · Paper 5 style · 4 marks
Lipase is added to full-cream milk containing a few drops of an indicator that is pink above pH 8.3 and colourless below it. A little sodium carbonate solution is first added so the milk starts pink. The time taken for the pink colour to disappear is recorded at four temperatures.temperature / °C | time for pink colour to disappear / s15 | 42025 | 21035 | 11055 | 600
(a)Explain why the pink colour disappears.[2]
(b)State the temperature at which lipase was most active in these results.[1]
(c)Calculate the rate of reaction at 25 °C as 1000 ÷ time. Give your answer to one decimal place.[1]
Show answer
(a) lipase digests fat in the milk to fatty acids (and glycerol); the fatty acids lower the pH below 8.3
B1 fats broken down to fatty acids; B1 acids lower the pH.
(b) 35 °C
B1.
(c) 4.8 (arbitrary units)
B1 1000 ÷ 210 = 4.8.
Q4Chemical digestion & enzymes · Standard · Paper 2 style · 1 mark
(a)Which row shows the places where starch is digested and the product of amylase action?[1]
Show answer
(a) B mouth and small intestine; maltose
Salivary amylase acts in the mouth and pancreatic amylase in the small intestine (duodenum); amylase produces maltose, which maltase then breaks down to glucose. Glucose is the product of maltase, not amylase; amylase does not act in the acid stomach.
Q5Chemical digestion & enzymes · Standard · Paper 4 style · 5 marks
(a)Complete the passage using suitable words.Starch is broken down to ______ by the enzyme amylase. This sugar is then broken down to ______ by the enzyme ______, which is found on the membranes of the epithelium lining the small intestine. Proteins are broken down to ______ by proteases such as pepsin and ______.[5]
Q6Chemical digestion & enzymes · Standard · Paper 4 style · 5 marks
(a)A student mixes salivary amylase with starch solution at different pH values at 37 °C. A drop of the mixture is tested with iodine solution every 15 s until it no longer turns blue-black. The results are:pH | time for starch to disappear / s4 | 3006 | 757 | 608 | 10010 | 600The rate of reaction can be found as 1000 ÷ time. Calculate the rate at pH 7 and at pH 4. Give each answer to one decimal place.[2]
(b)State the optimum pH of this amylase from these results.[1]
(c)Explain why the starch takes 600 s to disappear at pH 10.[2]
Show answer
(a) pH 7: 16.7; pH 4: 3.3
B1 1000 ÷ 60 = 16.7; B1 1000 ÷ 300 = 3.3.
(b) pH 7
B1.
(c) pH far from the optimum changes the shape of the active site of amylase; fewer enzyme–substrate complexes form so digestion is slow
B1 active site changes shape / some enzyme molecules denatured; B1 starch fits less often / fewer enzyme–substrate complexes.
Q7Chemical digestion & enzymes · Standard · Paper 6 style · 5 marks
A student fills a length of Visking (dialysis) tubing with a mixture of starch solution and amylase solution, ties both ends and places it in a boiling tube of distilled water at 37 °C. Every 10 minutes, samples of the water outside the tubing are tested with iodine solution and with Benedict's solution.time / min | iodine test on water outside | Benedict's test on water outside0 | orange-brown | blue10 | orange-brown | green20 | orange-brown | orange30 | orange-brown | brick red
(a)Explain the results of the Benedict's test.[3]
(b)Explain why the iodine test on the water outside stays orange-brown throughout.[1]
(c)Describe a suitable control for this experiment.[1]
Show answer
(a) amylase digests starch to maltose / reducing sugar; maltose molecules are small enough to diffuse through the tubing; more sugar outside over time so colour goes from blue to brick red
B1 starch digested to reducing sugar (maltose); B1 sugar is small and diffuses out through the tubing; B1 concentration outside increases with time (colour sequence).
(b) starch molecules are too large to pass through the tubing
B1.
(c) repeat with boiled (denatured) amylase / with water instead of amylase
B1.
Q8Chemical digestion & enzymes · Challenge · Paper 4 style · 5 marks
(a)State two functions of the hydrochloric acid secreted in the stomach.[2]
(b)Pepsin stops working when the stomach contents reach the duodenum. Explain why.[3]
Show answer
(a) kills pathogens / harmful microorganisms in food; gives the acidic pH that is optimum for pepsin
(b) bile (alkaline) neutralises the acid so pH rises; pepsin is far from its optimum pH; the shape of its active site changes so substrate no longer fits
B1 bile / alkaline secretion neutralises acid, pH rises; B1 pepsin has a low optimum pH; B1 active site changes shape / enzyme denatured / no enzyme–substrate complexes form.
Q9Chemical digestion & enzymes · Challenge · Paper 4 style · 3 marks
(a)Explain why proteins in a meal of chicken karahi must be digested before they can be used by body cells.[3]
Show answer
(a) proteins are large insoluble molecules that cannot pass through the wall of the small intestine into the blood; they are broken down to small soluble amino acids that can be absorbed; cells then use the amino acids to build their own proteins
B1 proteins are large / insoluble; B1 cannot be absorbed (cannot cross the epithelium / membranes); B1 amino acids are small and soluble so can be absorbed and then used to make new proteins.
Q10Chemical digestion & enzymes · Challenge · Paper 4 style · 4 marks
(a)In some people with cystic fibrosis, thick mucus blocks the duct that carries pancreatic juice to the duodenum. Suggest and explain two effects of this on digestion.[4]
Show answer
(a) less amylase, protease (trypsin) and lipase reach the duodenum, so starch, protein and fat are less digested; pancreatic juice is alkaline, so acid from the stomach is less neutralised and enzymes in the small intestine are not at their optimum pH
Two effects, each with explanation (2 × 2): less fat digested because less lipase (fatty food passes out in faeces); less protein / starch digested because less trypsin / amylase; fewer nutrients absorbed so poor growth / weight loss; contents of duodenum stay acidic so intestinal enzymes work slowly.
Q11Physical digestion, teeth & bile · Core · Paper 2 style · 1 mark
(a)What is the effect of bile on fats in the small intestine?[1]
Show answer
(a) B It emulsifies fats into small droplets.
Bile emulsifies fat, breaking large drops into small droplets and increasing their surface area for lipase. It contains no enzymes, so A is wrong: that is the action of lipase.
Q12Physical digestion, teeth & bile · Core · Paper 4 style · 4 marks
(a)Name the type of human tooth used for:(i) biting off pieces of food(ii) grinding and chewing food[2]
(b)Explain how chewing food helps chemical digestion.[2]
Show answer
(a) (i) incisors (ii) molars (accept premolars)
B1 each.
(b) chewing breaks food into smaller pieces, increasing the surface area for enzymes to act on, so digestion is faster
B1 increases surface area; B1 for enzymes / faster chemical digestion.
Q13Physical digestion, teeth & bile · Standard · Paper 4 style · 6 marks
(a)State where bile is made and where it is stored.[2]
(b)Explain two ways in which bile helps the digestion of fats in the small intestine.[4]
Show answer
(a) made in the liver; stored in the gall bladder
B1 each.
(b) emulsifies fats into small droplets, increasing surface area for lipase; it is alkaline and neutralises stomach acid, giving a suitable pH for enzymes in the small intestine
B1 emulsification; B1 larger surface area for lipase so faster digestion; B1 bile is alkaline / neutralises hydrochloric acid; B1 optimum pH for enzymes in the small intestine.
Q14Physical digestion, teeth & bile · Standard · Paper 4 style · 5 marks
(a)Describe how the stomach carries out physical digestion.[2]
(b)A cube of boiled potato with sides of 1 cm is cut into 8 equal cubes, each with sides of 0.5 cm. Calculate the total surface area before and after cutting.[2]
(c)Use your answer to (b) to explain why physical digestion speeds up chemical digestion.[1]
Show answer
(a) muscles in the stomach wall contract to churn the food, mixing it with gastric juice and breaking it into smaller pieces
B1 muscle contractions / churning; B1 breaks food up / mixes with juices.
(c) the surface area doubles, so more food is exposed to enzymes at once
B1.
Q15Physical digestion, teeth & bile · Challenge · Paper 4 style · 3 marks
(a)A patient has her gall bladder removed. Her liver continues to make bile, which now trickles slowly and continuously into the duodenum. Explain why the doctor advises her to avoid large fatty meals.[3]
Show answer
(a) without a store there is no large release of bile when a fatty meal arrives; fat is poorly emulsified, so surface area is small; lipase digests the fat slowly / incompletely, causing discomfort or fatty faeces
B1 bile cannot be released in large amounts at meal times; B1 less emulsification / small surface area of fat; B1 fat digestion by lipase is slow / incomplete.
Q16Physical digestion, teeth & bile · Challenge · Paper 6 style · 5 marks
A student investigates the effect of bile salts on fat digestion. Two test-tubes each contain 5 cm³ of vegetable oil mixed with 5 cm³ of a dilute alkaline solution containing an indicator. Tube A also has 1 cm³ of bile salt solution; tube B has 1 cm³ of distilled water. Lipase is added to both tubes at 35 °C and the time for the indicator to change colour (showing a fall in pH) is recorded.tube A (with bile salts): 4 mintube B (without bile salts): 11 min
(a)Calculate the percentage decrease in time when bile salts are present. Give your answer to two significant figures.[2]
(b)Explain why the reaction is faster in tube A.[2]
(c)Explain why 1 cm³ of distilled water was added to tube B.[1]
Show answer
(a) 64 %
M1 (11 − 4) ÷ 11 × 100; A1 64 %.
(b) bile salts emulsify the oil into small droplets; larger surface area for lipase so fatty acids form faster
B1 emulsification; B1 larger surface area for lipase / more enzyme–substrate collisions.
(c) to keep the total volume (and so concentrations) the same in both tubes
B1.
Q17Balanced diet & deficiencies · Core · Paper 2 style · 1 mark
(a)Which row correctly matches a nutrient with the disease caused by its deficiency?[1]
Show answer
(a) C vitamin D – rickets
Lack of vitamin D (and calcium) causes rickets; lack of vitamin C causes scurvy. A and B swap the two vitamins.
Q18Balanced diet & deficiencies · Core · Paper 4 style · 4 marks
(a)State what is meant by a balanced diet.[2]
(b)State two reasons why the body needs water.[2]
Show answer
(a) a diet containing all the nutrients (carbohydrates, fats, proteins, vitamins, minerals, fibre and water) in the correct proportions
B1 all nutrient groups; B1 in the correct amounts / proportions.
(b) solvent for transport (e.g. in blood plasma); medium for chemical reactions; cooling by sweating; removal of waste in urine
1 mark each for any two.
Q19Balanced diet & deficiencies · Standard · Paper 4 style · 4 marks
(a)A hostel student eats mostly white rice and bread with very little fruit, vegetables or meat. Suggest two nutrients that may be lacking in this diet and state a good source of each.[2]
(b)Explain why the body needs (i) iron and (ii) dietary fibre.[2]
Show answer
(a) e.g. vitamin C – citrus fruits / guava; iron – red meat / spinach; fibre – vegetables / whole grains; protein – meat / pulses; calcium – milk
1 mark each for nutrient with a matching source.
(b) (i) iron is needed to make haemoglobin in red blood cells; (ii) fibre helps food move through the gut / prevents constipation
B1 each.
Q20Balanced diet & deficiencies · Challenge · Paper 4 style · 5 marks
(a)100 g of guava contains 228 mg of vitamin C. A teenager needs 65 mg of vitamin C each day. Calculate the mass of guava, in g, that provides the daily need. Give your answer to two significant figures.[2]
(b)Name the deficiency disease caused by a long-term lack of vitamin C.[1]
(c)Explain why rickets is more common in children who rarely go outdoors and drink little milk.[2]
Show answer
(a) 29 g
M1 65 ÷ 228 × 100 (= 28.5…); A1 29 g.
(b) scurvy
B1.
(c) little sunlight on skin so less vitamin D made; little calcium (from milk) and vitamin D needed to absorb calcium, so bones are soft / weak
B1 vitamin D made in skin in sunlight / milk supplies calcium (and vitamin D); B1 calcium needed for strong bones, vitamin D helps calcium absorption.
Q21Digestive system organs & processes · Core · Paper 2 style · 1 mark
(a)What is the term for passing out of food that has not been digested or absorbed, as faeces, through the anus?[1]
Show answer
(a) C egestion
This is egestion. Excretion is the removal of metabolic waste (e.g. urea, CO₂); undigested food was never part of body cells.
Q22Digestive system organs & processes · Core · Paper 4 style · 5 marks
(a)State one function of each organ in digestion.(i) salivary glands(ii) stomach(iii) pancreas(iv) liver(v) large intestine (colon)[5]
Show answer
(a) (i) secrete saliva containing amylase; (ii) secretes pepsin / hydrochloric acid, churns food; (iii) secretes amylase, trypsin and lipase into the duodenum; (iv) makes bile; (v) absorbs water
B1 each.
Q23Digestive system organs & processes · Standard · Paper 4 style · 4 marks
(a)Define digestion.[2]
(b)Write the following in the order food passes through them:ileum, stomach, rectum, oesophagus, duodenum, colon[2]
Show answer
(a) the breakdown of large insoluble food molecules into small soluble molecules (using mechanical and chemical processes)
B1 large / insoluble to small / soluble; B1 so they can be absorbed (or mention of mechanical and chemical).
Q24Digestive system organs & processes · Standard · Paper 4 style · 5 marks
(a)Match each statement to one process: ingestion, digestion, absorption, assimilation or egestion.(i) amino acids are used by a muscle cell to make new proteins(ii) a child takes a bite of a samosa(iii) glucose moves from the ileum into the blood(iv) fibre leaves the body through the anus(v) starch is broken down to maltose in the mouth[5]
Q25Digestive system organs & processes · Challenge · Paper 4 style · 6 marks
(a)A student eats a meal of rice and daal (lentils). Rice is mainly starch and daal contains much protein. Describe what happens to the starch and protein from the meal as it passes from the mouth to the end of the ileum.[6]
Show answer
(a) mouth: chewing, salivary amylase starts starch → maltose; stomach: acid stops amylase, pepsin begins protein digestion, churning; duodenum: pancreatic amylase and trypsin continue; bile neutralises acid; maltase on epithelium makes glucose; proteases make amino acids; glucose and amino acids absorbed by villi into capillaries in the ileum
1 mark each for any six: chewing increases surface area; salivary amylase in mouth; starch to maltose; stomach acid denatures / stops amylase; pepsin digests protein in stomach; pancreatic juice into duodenum with amylase and trypsin; bile / alkaline juice neutralises acid; maltase converts maltose to glucose (on membranes of epithelium); protein digested to amino acids; absorption of glucose and amino acids in the ileum into blood capillaries of villi.
Q26Absorption, villi & assimilation · Core · Paper 4 style · 3 marks
(a)Describe three features of villi that increase the rate of absorption of digested food.[3]
Show answer
(a) microvilli give a large surface area; wall only one cell thick (short diffusion distance); dense capillary network maintains a concentration gradient; lacteal for fat absorption
1 mark each for any three.
Q27Absorption, villi & assimilation · Standard · Paper 2 style · 1 mark
(a)Into which structure in a villus are most fatty acids and glycerol absorbed?[1]
Show answer
(a) B lacteal
Fatty acids and glycerol pass into the lacteal, a lymphatic vessel at the centre of the villus. Glucose and amino acids enter the blood capillaries instead.
Q28Absorption, villi & assimilation · Standard · Paper 4 style · 4 marks
(a)In coeliac disease, eating gluten damages the lining of the small intestine and the villi become flattened. In one patient the absorptive surface area fell from 32 m² to 4 m². Calculate the percentage decrease in surface area. Give your answer to two significant figures.[2]
(b)Suggest why an untreated patient with coeliac disease may lose body mass even though they eat normally.[2]
Show answer
(a) 88 %
M1 (32 − 4) ÷ 32 × 100 (= 87.5); A1 88 %.
(b) smaller surface area means less glucose, amino acids and fats absorbed into the blood; less energy and fewer nutrients available, so body stores are used
B1 less absorption of digested food; B1 less energy / materials, so fat or protein stores are used up.
Q29Absorption, villi & assimilation · Challenge · Paper 4 style · 5 marks
(a)Define assimilation.[1]
(b)After absorption, glucose and amino acids travel to the liver. Describe what happens to them in the body after absorption.[4]
Show answer
(a) the movement of digested food molecules into the cells of the body where they are used, becoming part of the cells
B1.
(b) carried in the hepatic portal vein to the liver; glucose used in respiration to release energy or stored as glycogen in liver and muscles; amino acids used to make proteins (e.g. enzymes, plasma proteins); excess amino acids deaminated in the liver to form urea
1 mark each for any four: hepatic portal vein; glucose used for respiration; excess glucose converted to glycogen (stored in liver / muscles); amino acids used to build proteins; excess amino acids deaminated / urea formed in liver.
Q30Absorption, villi & assimilation · Challenge · Paper 4 style · 4 marks
(a)State where most water is absorbed in the alimentary canal.[1]
(b)Each day about 9.0 dm³ of water (from food, drinks and digestive juices) enters the small intestine. The small intestine absorbs 85 % of this water. The colon absorbs 90 % of the water that reaches it.Calculate the volume of water lost in the faeces each day, in cm³.[3]
Show answer
(a) small intestine
B1.
(b) 135 cm³
M1 water reaching colon = 9.0 × 0.15 = 1.35 dm³; M1 water left = 1.35 × 0.10 = 0.135 dm³; A1 135 cm³.
Similar questions
Questions that test the same subtopic in different years, grouped so you can work on one skill at a time.
References only: find each question in its paper (official link where available, otherwise through your school).
Chemical digestion & enzymesBanker67 questions · 9/9 sessions